| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1353775 | Biochemical Systematics and Ecology | 2016 | 5 Pages |
•26 new polynucleotide-repeat microsatellite loci were isolated in Lateolabrax maculatus.•Transferability of 6 loci was positive across 10 phylogenetically related species of Latidae and Serranidae.•These markers will help to study population genetics and conservation management.
The spotted sea bass Lateolabrax maculatus, a commercially important species of fishery resource in the Northwestern Pacific, has become a popular cultured fish species in China. Although the microsatellite markers of L. maculatus have been isolated, they mainly focused on dinucleotide-repeat loci. In this study, we developed 26 polynucleotide-repeat microsatellite loci for L. maculatus through high-throughput DNA sequencing technology. The number of alleles per locus ranged from 3 to 19. Observed and expected heterozygosities varied from 0.188 to 0.906 and 0.175 to 0.938, respectively. One locus showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction. These 26 novel microsatellite markers will serve as useful tools which benefit population genetics studies and conservation management of L. maculatus. In addition, cross-species amplification tests showed that the transferability of 6 loci was positive across 10 species of two phylogenetically related families Latidae and Serranidae, and 3 loci were successful amplified in at least 5 species. The transferable loci would be helpful for the detection of genetic relationship and divergence between the tested taxa.
