| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1354346 | Biochemical Systematics and Ecology | 2011 | 6 Pages |
Murraya koenigii (L.) Spreng., commonly known as curry leaf plant, is found in the different hilly regions of India. In the present study, fifty-nine accessions representing eight wild populations of M. koenigii were analyzed using thirteen ISSR primers. A total of 152 bands were amplified, out of which, 136 were polymorphic corresponding to 89.47% polymorphism across the accessions. The pairwise population genetic distances were calculated for all the populations that varied from 0.05 to 0.13 between the populations of M. koenigii. AMOVA and Nei’s genetic diversity analysis revealed higher genetic variations within populations than among the populations. The clustering of populations in the dendrogram was not in congruence with geographical affiliations. The results indicate that the ISSR method is sufficiently informative and powerful to estimate the genetic diversity in M. koenigii populations. As M. koenigii is an important wild plant genetic resource, therefore, information on genetic variability might be a potential source as breeding material for development of commercially valuable traits in M. koenigii plants.
► This work on Murraya koenigii populations occurring in natural state in India is an important account of the available genetic diversity. ► Inter Simple Sequence Repeat (ISSR) markers were employed to estimate genetic diversity within and among the wild populations of M. koenigii. ► Fifty-nine accessions representing eight populations were analyzed with thirteen ISSR primers, revealing 89.47% polymorphism across the accessions. ► AMOVA and Nei's genetic diversity analysis revealed higher genetic variations within populations than among populations. ► The results indicate that the ISSR method is sufficiently informative and powerful to estimate the genetic diversity in M. koenigii populations.
