Isoenzyme variation in two allopatric populations of Hedysarum pogonocarpum Boiss. (Fabaceae), a Turkish endemic

Of the four loci identified, ADH, MDH-1, MDH-2, PGM-2, only MDH-2 was monomorphic in both populations. The proportion of polymorphic loci in both populations of H. pogonocarpum was thus 75%, and the average allele number at each locus was 1.8. ADH isoenzymes in both populations were in the Hardy-Weinberg equilibrium, whereas for the MDH-1 locus there was more heterozygosity (P < 0.001) than expected. While the Kahramanmaras population was found to be in the Hardy-Weinberg equilibrium at the PGM-2 locus, the Malatya population displayed considerable deficiency (P < 0.01) in heterozygosity. About 10% of the total genetic variation was due to the interpopulational differentiation. The level of gene flow was found to be 2.05 per generation. Nei's genetic distance coefficient (0.112) and genetic identity (0.888) propose that two populations analysed could be accepted as subspecies of H. pogonocarpum.