| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1355166 | Bioorganic Chemistry | 2013 | 5 Pages |
Starch nanoparticles (StNPs) were acylated under ambient conditions to obtain various nanosized derivatives formed stable suspension in water and soluble in organic solvents. The degree of substitution (DS) was determined using 1H NMR technique. The cytotoxicity potential of the derivatised StNPs was evaluated in mouse embryonic fibroblast (3T3L1) cells and A549 tumor cell line using MTT cell viability assay. Other parameters that determine the oxidative stress viz., reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), superoxide generation and acridine orange/ethidium bromide staining were also investigated. The present study led to the conclusion that cytotoxic activity of acylated starch nanoparticles was dependent on their dosage, DS and type of substitution. The non-toxic nature in non-cancerous cells reveals that the nanoparticles (NPs) can be used for cancer therapy and drug delivery. The nanoparticles also offered reasonable binding propensity with CT-DNA.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Nanosized acyl derivatives of starch synthesized and characterized. ► Cytotoxic potential of acylated starch nanoparticles reported for the first time. ► Despite negative zeta potential good biding propensity with CT-DNA was exhibited. ► Noncytotoxicity in non-cancerous cells suggest promising drug delivery application.
