Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1356016 | Bioorganic Chemistry | 2009 | 9 Pages |
The paper describes ex vivo applications of colchicine derivatives for the treatment of human T-Lymphoblastoid (CEM) cells. Moreover, the role of the substitutions of ring A at C-1 and C-7 side chain of colchicine analogues was probed by the synthesis and examination of their effects on the three-dimensional (3-D) CEM cells’ growth. The CEM cells were cultured in the hollow fiber bioreactor (HFB) device. We used 1H and 19F magnetic resonance imaging (MRI) to monitor changes in 3-D CEM cell culture. 19F MRI was used for visualization of the cellular uptake of new fluorine derivatives. Before and after treatment CEM cells profile was investigated with high performance liquid chromatography (HPLC-UV).
Graphical abstract19F MR image showed fluorine derivative uptake in CEM cells cultured in hollow fiber bioreactor.Figure optionsDownload full-size imageDownload as PowerPoint slide