Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1375826 | Carbohydrate Polymers | 2014 | 5 Pages |
•New strain Aspergillus versicolor was isolated for maximum production of cellulose degrading enzyme.•Cellulose degradation was performed using agar plate method.•Variation of a single parameter at a time method was used in CMC containing medium.•Aspergillus versicolor showed maximum production of CMCase at 30 °C.
Production of CMCase from newly isolated strain of Aspergillus versicolor was studied by optimizing different physico-chemical parameters. Cellulolytic potential of the isolate was confirmed by the simple agar plate method and then subjected to variation of a single parameter at a time in CMC containing medium. Maximum enzyme production was obtained at 30 °C in 120 h when medium was supplemented with 0.5 gm% carboxymethyl cellulose, 0.2 gm% Tween 80, 0.075 gm% peptone, 0.050 gm% CaCl2, 1.5 gm% NaNO3 and 0.2 gm% KH2PO4 at pH 4.0. Current study holds great potential as the organism reported here could easily be used for the production of cellulase using renewable biomass.