Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1383062 | Carbohydrate Polymers | 2016 | 11 Pages |
•A longer reagent infiltration time increased reaction uniformity within granules.•Amylose (AM) was reacted more than amylopectin (AP) for all infiltration times.•AM:AP reactivity increased with increasing granular reaction uniformity (wheat starch).•Pasting viscosity decreased (cross-linking) as the reagent infiltration time increased.•Granular/molecular reaction locale impacts pasting properties of starch derivatives.
The impact of granular and molecular reaction patterns on modified starch properties was investigated as a function of the length of time allowed for reagent to infiltrate starch granules. A fluorescent reagent [5-(4,6-dichlorotriazinyl)aminofluorescein] was dispersed in aqueous normal maize or wheat starch slurries (35%, w/v) for 0, 5, 10, 30, or 60 min, after which reaction was initiated by increasing the pH to 11.5 and allowing reaction to proceed for 3 h. With increasing lengths of infiltration, the reaction became increasingly homogeneous within the granule interior (matrix) and the AM:AP reactivity ratio increased (wheat starch), as assessed by confocal laser scanning microscopy (CLSM) and size-exclusion chromatography (refractive index and fluorescence detection), respectively. A longer reagent infiltration time also led to a more inhibited (i.e., cross-linked) pasting viscosity, suggesting that both granular and/or molecular reaction patterns were altered by varied reagent infiltration times to ultimately impact modified starch properties.