| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1383106 | Carbohydrate Polymers | 2016 | 11 Pages |
•The hot water extraction of CHPS was optimized by a Box–Behnken design.•The maximum extraction yield of CHPS was 5.37 ± 0.15%.•Three fractions were purified from CHPS by using the method of chromatography.•Crude CHPS and its purified fractions showed excellent antioxidant activity in vitro and in cellular level.
The optimal extraction conditions with a yield of 5.37 ± 0.15% for extraction of polysaccharides from chickpea (Cicer arietinum L.) hull (CHPS) were determined as extraction temperature 99 °C, extraction time 2.8 h and ratio of water to raw material 24 mL/g. Three fractions of CHPS-1, CHPS-2 and CHPS-3, with average molecular weight of 3.1 × 106, 1.5 × 106 and 7.8 × 105 Da, respectively, were obtained from crude CHPS by chromatography of DEAE Fast Flow and Sephadex G-100. CHPS-1 was composed of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose, CHPS-2 was composed of mannose, rhamnose, galacturonic acid, galactose, xylose and arabinose, CHPS-3 was composed of galacturonic acid, galactose and rhamnose. CHPS-3 showed the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and highest scavenging activities against DPPH and ABTS radicals, while CHPS-2 showed the highest scavenging activity against superoxide anion radical.
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