Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1383471 | Carbohydrate Polymers | 2016 | 12 Pages |
•X-ray analysis of β-CD inclusion complexes with (+)-catechin and (−)-epicatechin.•Two complexes exist in three crystal forms, monoclinic, P21 and triclinic, P1.•OH⋯O H-bonds and π⋯π intermolecular interactions stabilize the complexes.•Rare A-conformer and dimeric structure of tea catechins in the cavity confinement.•Number of OH groups protected play a key role in antioxidant activity improvement.
Single-crystal X-ray analysis to 0.6 Å resolution using synchrotron radiation of the inclusion complexes of β-cyclodextrin (β-CD) with (+)-catechin (CA) and (−)-epicatechin (EC) has been carried out to gain atomistic insight into how the inclusion complexation helps to improve tea catechin antioxidant activity. The β-CD–CA complex crystallizes in the monoclinic P21 space group, whereas the β-CD–EC complex crystallizes in both the monoclinic P21 and triclinic P1 space groups. In comparing the inclusion structures, the β-CD–EC dimeric structure having higher numbers of OH⋯O hydrogen bonds combined with π⋯π interactions is associated with its greater interaction energy, as derived from DFT/B3LYP single-point energy calculations. Detailed structural analysis of the CA, EC conformation and inclusion geometry reveals that the interplay of the intermolecular OH⋯O hydrogen bond stabilized catechol moiety and the protection of OH groups in the β-CD cavity plays a key role in the improvement of antioxidant capacity upon inclusion complexation.
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