| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1383820 | Carbohydrate Research | 2013 | 5 Pages |
•Helicobacter pylori is the most common human pathogen.•Lipopolysaccharide of H. pylori is its virulence factor and can be used for vaccine preparation.•Previously published structure contained incorrect details.•New structure is presented, based on the use of modern methods.
In this study, we describe a re-investigation of the lipopolysaccharide structure of Helicobacter pylori serogroup O:3. Application of NMR and MS approaches to the analysis of oligosaccharides obtained through degradation of LPS from H. pylori serogroup O:3 by various methods confirmed that its general architecture was identical to that of LPS from H. pylori strains 26695 and SS1 and followed a sequential linear assembly of the α-1,6-glucan, dd-heptan, and O-chain components. Additionally, MALDI-MS analysis demonstrated that a significant proportion of H. pylori serogroup O:3 LPS was terminated with α-1,6-glucan and was not further substituted by dd-heptan and the O-chain polysaccharide.
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