Optimization of extraction technology of Se-enriched Hericium erinaceum polysaccharides by Box–Behnken statistical design and its inhibition against metal elements loss in skull

This article described the application of response surface methodology (RSM) to the development of a procedure for extraction of Se-enriched Hericium erinaceum polysaccharides. A Box–Behnken matrix was used to find optimal conditions for the procedure through a response surface study. Four variables “extraction time; extraction temperature; ratio of liquid to solid and extraction number” were regarded as factors in the optimization study. The optimal extraction conditions for the Se-enriched H. erinaceum polysaccharides were: extracting time 115 min, extracting temperature 98 °C, ratio of liquid to solid 4, and extracting number 4. Compared with untreated cells, administration of Se-enriched H. erinaceum polysaccharides improved BMC and BMD in experimental rats. These results showed that Se-enriched H. erinaceum polysaccharides could inhibit metal elements loss in rats’ skull.