Article ID Journal Published Year Pages File Type
1384353 Carbohydrate Polymers 2013 6 Pages PDF
Abstract

A homogenous extracellular polysaccharide, designated AWP, was isolated from the fermented liquid of the marine fungus Aspergillus versicolor from the coral Cladiella sp. and purified by anion-exchange and size-exclusion chromatography (SEC). Chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopy showed that AWP consisted of glucose and mannose in a molar ratio of 8.6:1.0, and its average molecular weight was estimated to be 500 kDa. AWP is a slightly branched extracellular polysaccharide. The backbone of AWP is mainly composed of (1→6)-linked α-d-glucopyranose residues, slightly branched by single α-d-mannopyranose units attached to the main chain at C-3 positions of the glucan backbone. The investigation demonstrated that AWP is a novel extracellular polysaccharide different from those of other marine microorganisms.

► AWP was obtained from the fermented liquid of Aspergillus versicolor. ► Structure of AWP was elucidated by GC–MS, FTIR and NMR. ► The backbone of AWP consisted of glucopyranose units. ► The side chain contained mannopyranose units. ► AWP was a novel polysaccharide different from other exopolysaccharides.

Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
Authors
, , , , , , , , , , , , ,