Modelling of xyloglucan, pectins and pectic side chains binding onto cellulose microfibrils

Binding modelling of tamarind and pea xyloglucans, sugar beet and potato pectins, and pectic side chains (branched arabinan, debranched arabinan, galactan) onto microcrystalline Avicel cellulose and primary cell wall (PCW) cellulose was performed. The most commonly used binding models, namely the Langmuir, the Freundlich and the Scatchard models, were applied to the data. It appeared that the Freundlich model was more appropriate to describe the binding of all the polysaccharides used in this study. The heterogeneity index calculated from the slope of Freundlich isotherms highlights an important heterogeneity of Avicel and PCW cellulose surfaces, in agreement with the Scatchard representation.