Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1384636 | Carbohydrate Research | 2010 | 6 Pages |
Abstract
To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of GlcNAc were found to be essential for the transglycosylation reaction using Endo-M. Furthermore, it was determined that Endo-M strictly recognizes a 1,3-diol structure consisting of primary and secondary hydroxyl groups.
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Related Topics
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Authors
Yusuke Tomabechi, Yuki Odate, Ryuko Izumi, Katsuji Haneda, Toshiyuki Inazu,