| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1384707 | Carbohydrate Polymers | 2016 | 10 Pages |
•One amido bond formed between C-terminal carboxyl of ES2 and C-2-NH2 of HTCOSC.•Improved heat stability was obtained through the conjugation of HTCOSC to ES2.•Better bioactivities of HTCOSC-ES2 were obtained both in vitro and in vivo.•HTCOSC-ES2 has good potential in angiogenesis related diseases treatment.
Soluble O-(2-hydroxyl)propyl-3-trimethyl ammonium chitooligosaccharide chloride (HTCOSC) was covalently conjugated to the 11-amino-acid peptide derived from amino terminus of endostatin (endostatin2, ES2, IVRRADRAAVP) to overcome its poor stability, low cell affinity and instable activity. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to study the structure and molecular weight information. The anti-angiogenic activities were evaluated using cell counting Kit-8 assay, flow cytometry assay, wounding migration assay, transwell migration assay, chicken chorioallantoic membrane (CAM) assay and zebra fish angiogenesis assay. In contrast with ES2, the novel carbohydrate-polymer HTCOSC-ES2 displayed improved heat stability, higher cell affinity, better inhibition on endothelial cell proliferation, tube formation, 2-dimensional and 3-dimensional migration in vitro. According to the evaluation in CAM and zebra fish, HTCOSC-ES2 also displayed better anti-angiogenic activity than ES2 in vivo. These results indicate that HTCOSC has good properties as potential candidate for protein/peptide modifier and HTCOSC-ES2 has good potential in angiogenesis related diseases treatment.
