Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1384757 | Carbohydrate Research | 2010 | 7 Pages |
Abstract
A combination of recombinant FKP and α-(1→3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5′-diphosphate-β-l-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
David Soriano del Amo, Wei Wang, Christen Besanceney, Tianqing Zheng, Yizheng He, Brian Gerwe, Ronald D. Seidel III, Peng Wu,