Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1384913 | Carbohydrate Polymers | 2008 | 6 Pages |
As polysaccharides of the mushroom genus Pleurotus can act as biological response modifiers, we now describe the chemical characterization of a new water-soluble αβ-glucan from the fruiting bodies of the edible mushroom Pleurotus florida. It was obtained via successive aq. extraction, freeze-thawing, precipitation with Fehling solution, and dialysis through a membrane with Mr 1000 kDa cut-off. It was analyzed using NMR spectroscopy, HPSEC, monosaccharide composition, methylation analysis, and a controlled Smith degradation. It was a branched glucan (Mw 1.1 × 106 g/mol), with a main-chain of (1→3)-linked-α-d-Glcp residues, partially substituted at O-6 by single-unit β-d-Glcp, and a minor proportion by side-chains with 3-O-substituted β-d-Glcp groups.