Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1384951 | Carbohydrate Polymers | 2012 | 9 Pages |
A novel class of complex oligosaccharides with a backbone made of twelve β-(1→4)-d-glucosyl units that may be regularly substituted on their primary position with α-d-xylosyl or β-d-(1→2)-galactosyl-α-d-xylosyl residues were readily synthesized using enzymatic hydrolysis of the plant polysaccharide xyloglucan (XG), followed by chemical modifications of the well-defined oligosaccharides and their enzymatic coupling catalyzed by the Cel7B E197A glycosynthase from Humicola insolens. These complex oligosaccharides proved useful for a better understanding of the effects of structural variation of xyloglucan on interactions with bacterial microcrystalline cellulose (BMCC).
► Cellosynthase was used as catalyst for the preparation of xylogluco-oligosaccharides. ► Pure xylogluco-oligosaccharides and cellulose interactions were evaluated. ► Affinity was evaluated by adsorption isotherm.