| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1385240 | Carbohydrate Polymers | 2007 | 7 Pages |
Abstract
Using d-glucosamine hydrochloride (GlcNH2·HCl) as starting material, a new amino acid sugar conjugate, arginine-glucosamine (Arg-GlcNH2), was synthesized and characterized by infrared spectroscopy, 13C NMR and element analysis. Its cytotoxicity in vitro was evaluated by MTT assay. The inhibition ratio against human hepatoma cell SMMC-7721 was higher than that of GlcNH2·HCl. This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry. In addition, SMMC-7721 cells treated with Arg-GlcNH2 resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis. The manner of Arg-GlcNH2 cytocidal activity was concluded to be apoptosis.
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Authors
Li Zhang, Wanshun Liu, Baoqin Han, Dongfeng Wang,