Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1385301 | Carbohydrate Polymers | 2011 | 8 Pages |
Box–Behnken design was employed to optimize the ratio of water to raw material (X1: 20–40 mL/g), extraction time (X2: 20–40 min) and extraction temperature (X3: 40–60 °C) to obtain a high crude polysaccharides yield from Tremella mesenterica (TMP) by ultrasonic-assisted extraction technique (UAE). The optimum conditions were a ratio of water to raw material of 34 mL/g and extraction time of 30 min at 50 °C. Under these conditions, the experimental yield was 8.26%, well matched with the predicted models with the coefficients of determination (R2) of 0.9918. The crude TMP was purified by Sephadex G-100 chromatography, giving two major fractions (TMPA and TMPB), which were composed of xylose, mannose and glucose in molar ratio of 1.00:4.74:0.91 and 1.00:6.63:2.34, respectively. The evaluation of antioxidant activity suggested that the polysaccharides exhibited significant protection against hydrogen peroxide induced oxidative damage in rat erythrocytes and could be explored as a nutraceutical agent.