Article ID Journal Published Year Pages File Type
1385850 Carbohydrate Polymers 2014 10 Pages PDF
Abstract

•A hyperbranched heteropolysaccharide was isolated from natural Cordyceps sinensis.•Methylation analysis of polysaccharide was performed under microwave irradiation.•SEC-MALLS and SEC-TDA were applied for determination of hydrodynamic characterization of polysaccharide.•The chain conformation of polysaccharide from natural C. sinensis was firstly provided.

A polysaccharide, named as cordysinan, extracted from natural Cordyceps sinensis, was identified as a hyperbranched heteropolysaccharide from the results of FT-IR, GC–MS, and carbohydrate analysis by carbohydrate gel electrophoresis analysis, as well as the degree of branching of cordysinan was 43.3%. The solution properties of cordysinan were investigated by using size exclusion chromatography coupled with multi-angle laser light scattering and triple detector array, respectively. The molecular weights, the radius of gyration and the intrinsic viscosity of cordysinan were determined as 22.45 ± 0.26 kDa and 22.37 kDa, 15.4 ± 2.4 nm and 1.41 mL/g, respectively. By applying the polymer solution theory, the exponent (ν and α  ) values of S2z1/2=kMwν and [η]=kMwα were calculated as 0.28 and 0.42, respectively, which firstly revealed that cordysinan existed as a globular shape in 0.9% NaCl aqueous solution. Moreover, the results showed that cordysinan could obviously stimulate macrophages functions.

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Physical Sciences and Engineering Chemistry Organic Chemistry
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