Complete 1H and 13C NMR assignment of digeneaside, a low-molecular-mass carbohydrate produced by red seaweeds

Digeneaside (α-d-mannopyranosyl-(1→2)-d-glycerate) was extracted from the red algae, Bostrychia binderii, and purified by adsorption and gel-filtration chromatography. HPLC and ESI-MS techniques were used to follow purification steps and characterize digeneaside. NMR spectroscopy experiments (1D 1H, 13C, DEPT and 2D HMQC, COSY and TOCSY) were used to fully assign the 1H and 13C spectra.

Graphical abstractDigeneaside (α-d-Manp-(1→2)-d-glycerate) was extracted from the red algae, Bostrychia binderii. HPLC and ESI-MS techniques were used to characterize the glycoside. NMR experiments (1D 1H, 13C, DEPT and 2D HMQC, COSY and TOCSY) were used to fully assign the 1H and 13C spectra.Figure optionsDownload full-size imageDownload as PowerPoint slide