| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1386540 | Carbohydrate Polymers | 2011 | 7 Pages |
In this work, the effects of submerged culture conditions and nutritional requirements on simultaneous production of mycelial biomass and intracellular polysaccharide (IPS) from a medicinal mushroom Fomes fomentarius were studied using desirability functions. Under the optimal culture condition, the production of mycelia and IPS reached 17.19 and 2.86 g l−1, respectively in a 15l stirred tank bioreactor, which were about twice than that of the basal medium. Furthermore, the ethanol extract of mycelia (EEM) and IPS had a direct antiproliferative effect on human gastric cancer cell lines SGC-7901 and MKN-45 in a dose-dependent manner. In contrast, human normal gastric cell line GES-1 was less susceptible to EEM and IPS. These results suggest that F. fomentarius may represent a promising novel approach for gastric cancer intervention.
