Some findings in transgalactosylations employing modified donor substrates

•β-Galactosidase (bovine testis).•Transgalactosylation.•Modified p-nitrophenyl glycopyranosides.•Modified T-antigen components.

The scope of transgalactosylation with β-galactosidase (bovine testis) was studied employing a series of modified donor substrates based on p-nitrophenyl β-d-galactopyranoside and as uniform acceptor allyl 2-N-acetamido-2-deoxy-α-d-galactopyranoside. Structurally diverse donor molecules were recognized by the enzyme and led to novel disaccharide components, yet an excessive structural distortion was not accepted.

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