Article ID Journal Published Year Pages File Type
1387617 Carbohydrate Research 2014 11 Pages PDF
Abstract

•We report a comparative glycopeptides assay control vs. pathological urine.•Chip-based nanoESI QTOF MS was used for mapping and sequencing.•No fucosylated species have been identified in healthy urine.•An increased level of fucosylation is reported for pathological urine.•Fucosylated species Thr-linked were for the first time structurally characterized.

In this study an integrative mass spectrometry (MS) approach based on fully automated chip-nanoelectrospray quadrupole time-of-flight was optimized and applied for the discovery and structural characterization of O-glycopeptides in a fraction from the urine of a patient diagnosed with Schindler disease type I. A mixture of O-glycopeptides extracted and purified from an age matched healthy subject served as the control. 49 glycoforms were discovered in the investigated urine fraction from Schindler disease versus only 14 in control urine. Structures with relevant biological significance, previously not described, such as O-fucosylated tetrasaccharides and chains up to pentadecamers O-linked to serine, threonine, or threonine-proline were identified in the pathological urine and characterized by tandem MS (MS/MS). A number of 29 species discovered here, most of which with long chain glycans, were not previously reported as associated to this condition. All glycopeptides were detected in only 1 min analysis time, with a sample consumption situated in the femtomole range.

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Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
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