Structural characterization and gastroprotective property of a novel glucofructan from Allium ampeloprasum var. porrum

•A polysaccharide was isolated from Allium ampeloprasum var. porrum.•The chemical structure was established by spectroscopic techniques and chemical conversions.•The gastroprotective property was investigated using in vivo experimental models.

A new polysaccharide with an estimated weight-average molar mass of 2.6 × 103 was isolated from Allium ampeloprasum var. porrum by hot water extraction, and purified by Sephacryl S-300 HR high-resolution chromatography. It was composed of d-fructose and d-glucose in 10:6 molar ratio, respectively. The structure of the glucofructan was investigated by chemical and spectroscopic methods, including methylation analysis, nuclear magnetic resonance, and electrospray mass spectrometry (ES-MS). The results permitted the structure of the glucofructan to be written as α-d-Glcp-(1→1)-β-d-Fruf-(2→1)-{[α-d-Glcp-(1→6)-β-d-Fruf-(2→6)]-β-d-Fruf-(2→1)}4-β-d-Fruf-(2↔1)-α-d-Glcp. Results of the present study indicated that this new glucofructan exhibited significant gastroprotective property, using in vivo experimental models.

Graphical abstractα-d-Glcp-(1→1)-β-d-Fruf-(2→1)-{[α-d-Glcp-(1→6)-β-d-Fruf-(2→6)]-β-d-Fruf-(2→1)}4-β-d-Fruf-(2↔1)-α-d-Glcp.Figure optionsDownload full-size imageDownload as PowerPoint slide