| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1387883 | Carbohydrate Research | 2014 | 5 Pages |
•Specific, fluorometric enzyme-coupled assay for the measurement of cellulase is reported.•The synthesis of the fluorometric substrate is described.•Benzylidene acetal functionalisation prevents action of the exo-glycosidase present.•4-Methylumbelliferyl functionalisation provides fluorogen for simple quantitative detection.•Substrate can also be employed as a highly sensitive probe for metagenomic cellulase screening.
There is a growing demand for research tools to aid the scientific community in the search for improved cellulase enzymes for the biofuel industry. In this work, we describe a novel fluorometric assay for cellulase (endo-1,4-β-glucanase) which is based on the use of 4,6-O-benzylidene-4-methylumbelliferyl-β-cellotrioside (BzMUG3) in the presence of an ancillary β-glucosidase. This assay can be used quantitatively over a reasonable linear range, or qualitatively as a solution screening tool which may find extensive use in the area of metagenomics.
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