| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1388038 | Carbohydrate Research | 2013 | 7 Pages |
Chitooligosaccharides with degrees of polymerization (DPs) 2–12 were successfully separated and characterized using LC–MS/MS analysis with an amine column and a quadrupole ion trap analyzer. Major MS peaks for each chromatographic peak originated from deacetylated chitooligosaccharides while N-acetylated chitooligosaccharides were observed as minor peaks. The ratio of acetylation per glucosamine unit for each chitooligomer was higher for oligomers with higher DPs. The MS/MS spectra of chitooligosaccharides were used to characterize the chitooligosaccharide fragmentation patterns. To the best of our knowledge, this is the first report of on-line LC–MS/MS analysis of chitooligosaccharides with DP higher than 6.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Chitooligosaccharides were successfully analyzed using LC–MS/MS. ► Chitooligosaccharides with DPs from 2 to 12 were characterized. ► Major MS peaks for each chromatographic peak from deacetylated chitooligosaccharides. ► MS/MS spectra of chitooligosaccharides used to characterize fragmentation patterns.
