Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1388064 | Carbohydrate Research | 2010 | 5 Pages |
Abstract
Low concentrations of urea and GuHCl (2 M) enhanced the activity of endoglucanase (EC 3.1.2.4) from Aspergillus aculeatus by 2.3- and 1.9-fold, respectively. The Km values for controls, in the presence of 2 M urea and GuHCl, were found to be 2.4 ± 0.2 Ã 10â8 mol Lâ1, 1.4 ± 0.2 Ã 10â8 mol Lâ1, and 1.6 ± 0.2 Ã 10â8 mol Lâ1, respectively. The dissociation constant (Kd) showed changes in the affinity of the enzyme for the substrate with increases in the Kcat suggesting an increased turnover number in the presence of urea and GuHCl. Fluorescence studies showed changes in the microenvironment of the protein. The increase in the activity of this intermediate state was due to conformational changes accompanied by increased flexibility at the active site.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Gajendra S. Naika, Purnima Kaul Tiku,