Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1388365 | Carbohydrate Research | 2009 | 6 Pages |
Abstract
The binding of 2â²-deoxyuridine to human serum albumin (HSA) was investigated by fluorescence spectroscopy in combination with molecular modeling under simulation of physiological conditions. The quenching mechanism was suggested to be static according to the fluorescence measurement. The thermodynamic parameters: enthalpy change (ÎH) and entropy change (ÎS) were calculated to be â18.87 kJ/mol and 24.00 J/(mol K) according to the Vant'Hoff equation. These data suggest that hydrophobic interactions are the predominant intermolecular forces stabilizing the complex. Experimental results are in agreement with the results obtained by molecular modeling study. In addition, the effects of common ions on the binding constants were also studied at room temperature.
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Authors
Fengling Cui, Yinghua Yan, Qiangzhai Zhang, Juan Du, Xiaojun Yao, Guirong Qu, Yan Lu,