Chemoenzymatic synthesis of lacto-N-tetrasaccharide and sialyl lacto-N-tetrasaccharides

•Highly efficient one-pot two-enzyme large-scale synthesis of lacto-N-biose (LNB).•Convergent chemical synthesis of LNT using enzymatic generated LNB disaccharide building block.•Enzymatically synthesized building blocks for chemical synthesis with additional enzymatic modifications.

A concise and practical chemoenzymatic synthesis of lacto-N-tetraose, a major component and one of the most common core structures of human milk oligosaccharides (HMOs) was reported. This convergent synthesis relies on the glycosylation of a readily available lactoside acceptor with a lacto-N-biose donor generated from a highly efficient one-pot two-enzyme synthesis.

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