Identification of cell-surface mannans in a virulent Helicobacter pylori strain

With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations:In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host–microbial interactions and immunological modulation.

Graphical abstractThe structure of cell-surface mannans obtained from the virulent strain Helicobacter pylori 968 is as follows:Figure optionsDownload full-size imageDownload as PowerPoint slideThis strain also expressed cell-surface the emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation.