| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1389146 | Carbohydrate Research | 2011 | 4 Pages |
An O-polysaccharide and oligosaccharides were isolated by GPC following mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O28. The O-polysaccharide was studied by sugar and methylation analyses, 1H and 13C NMR spectroscopy, including 2D ROESY and H-detected 1H,13C HSQC and HMBC experiments, and the following structure of the branched pentasaccharide repeating unit was established:This structure was confirmed by ESI MS of the isolated tridecasaccharide consisting of the lipopolysaccharide core and one O-polysaccharide repeat. The ESI mass spectrum also enabled inferring the composition of the core oligosaccharide.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We isolated a lipopolysaccharide from a strain of Providencia alcalifaciens O28. ► Carbohydrates were released by mild acid delipidation of the lipopolysaccharide. ► The O-polysaccharide was studied by chemical methods and 2D NMR spectroscopy. ► The core and O-unit-bearing core oligosaccharides were studied by ESI MS. ► A new structure of a pentasaccharide repeat of the O-polysaccharide was established.
