Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465

The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacteriumbifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D 1H–1H homonuclear and 1H–13C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure:Figure optionsDownload full-size imageDownload as PowerPoint slideThe second isolated polysaccharide is a branched glucan with the main chain of (1→6)-linked α-d-glucopyranose residues, ∼60% of which are 2-substituted with a single α-d-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.

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