| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1389704 | Carbohydrate Research | 2008 | 15 Pages |
Abstract
The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1→4, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows:Figure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Sander S. van Leeuwen, Slavko Kralj, Ineke H. van Geel-Schutten, Gerrit J. Gerwig, Lubbert Dijkhuizen, Johannis P. Kamerling,