| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1390066 | Carbohydrate Research | 2016 | 6 Pages |
•The nuclear magnetic resonance spectral assignment of a bisecting hybrid-type oligosaccharide, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M).•The complete NMR assignment of a bisecting hybrid-type oligosaccharide through 1H- and 13C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments.•The determination of the structure of the glycoside linkage formed by the Endo-M transglycosylation.
This study describes the complete nuclear magnetic resonance (NMR) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M). Through 1H- and 13C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments, we determine the structure of the glycoside linkage formed by the Endo-M transglycosylation, i.e., the connection between GlcNAc and GlcNAc in oligosaccharide 1.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
