Structure of a zwitterionic O-polysaccharide from Photorhabdus temperata subsp. cinerea 3240

•An O-polysaccharide was isolated from Photorhabdus temperata subsp. ≿inerea 3240.•Structure of the polysaccharide was established by NMR and chemical approaches.•The polysaccharide contains d-FucNAc4N and d-GalNAc 4-(2-aminoethyl phosphate).•The polysaccharide possesses multiple acidic and basic functions in each repeat.•The structure established is not related to the structures found in other Photorhabdus species.

A phosphorylated O-polysaccharide was isolated from the lipopolysaccharide of an entomopathogenic bacterium Photorhabdus temperata subsp. cinerea 3240 and studied by sugar analysis, dephosphorylation, and 1H and 13C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit of the O-polysaccharide was established:→3)-β-d-GalpNAc4PEtN-(1→4)-β-d-GlcpA-(1→3)-β-d-FucpNAc4N-(1→where GlcA indicates glucuronic acid, FucNAc4N 2-acetamido-4-amino-2,4,6-trideoxygalactose, and PEtN 2-aminoethyl phosphate.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide