Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1390313 | Carbohydrate Research | 2013 | 6 Pages |
•Сell-wall phosphate-containing glycopolymers of B. longum were isolated.•A poly(oligosaccharyl phosphate), ribitol and glycerol teichoic acids were identified.•A GlcNAc/Glc (major/minor) alternation occurs in the poly(oligosaccharyl phosphate).•The main chain of the ribitol teichoic acid is terminated with d-alanine.
Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: Figure optionsDownload full-size imageDownload as PowerPoint slide The ribitol teichoic acid also contains minor d-alanine, whose position was not determined.
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