Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174

•The O-polysaccharide (OPS) of E. coli O174 was isolated from a mastitis-diseased cow.•Its chemical structure was determined utilizing 1D and 2D NMR spectroscopy.•The described here OPS structure of mastitis isolate differs from OPS structure of E. coli O174 human isolate.

Escherichia coli is one of the major causative agents of bovine mastitis, a disease affecting dairy herds. The lipopolysaccharide (LPS) of E. coli was plays a prominent role during infection. Here, we report on the O-antigen chemical structure of the LPS from Escherichia coli strain 2188 (serotype O174:H28) isolated from a mastitis-diseased cow. The structure of the OPS was analyzed by 1 and 2D NMR spectroscopy, and methylation analysis, which identified the branched repeating tetrasaccharide biological unit.Figure optionsDownload full-size imageDownload as PowerPoint slide

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