| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1390319 | Carbohydrate Research | 2013 | 4 Pages |
Abstract
The Cronobacter spp. are Gram-negative bacterial pathogens that can cause infections in all age groups, and have a high mortality rate in neonates due to necrotizing enterocolitis and meningitis. Recent genotyping studies have revealed a strong clonal lineage in the genus, but this has not been compared with physiological traits. The O-polysaccharides (OPS) were isolated from three C. turicensis sequence type 5 strains (57, 564, and 566) and structurally characterized using 1H and 13C NMR spectroscopy, including two-dimensional DQF-COSY, TOCSY, ROESY, and HSQC analysis. Further compositional determination was undertaken using classical chemical methods followed by GLC, and GLC-MS analysis. The repeating unit of the isolated O-polysaccharides consists of GlcNAc, Rha, Glc, and had the structure shown below and therefore complemented the sequence type.
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Authors
MaÅgorzata Czerwicka, Kinga Marszewska, Stephen J. Forsythe, Anna Bychowska, Alicja Mazgajczyk, Halina Dziadziuszko, Karolina Ossowska, Piotr Stepnowski, Zbigniew KaczyÅski,