Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1390614 | Carbohydrate Research | 2010 | 6 Pages |
Abstract
Basic treatment of a biantennary complex-type sialyloligosaccharide, as well as its asialo form, was found to lead to the specific cleavage of 2-acetamido-2-deoxy-d-glucose (GlcNAc) from the reducing end. The resultant oligosaccharides were identical to those prepared by treatment with endo-β-glycosidase-M, which cleaves the glycosidic bond between two GlcNAc residues at the reducing end of N-linked oligosaccharides. In addition, mechanistic studies suggested that an elimination reaction in the reducing-end terminal GlcNAc residue causes this specific cleavage reaction.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Takefumi Murase, Yasuhiro Kajihara,