| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1390859 | Carbohydrate Research | 2007 | 4 Pages |
Abstract
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant β-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13C NMR spectroscopy.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jingbao Li, Feng Han, Xinzhi Lu, Xiaoyan Fu, Cuiping Ma, Yan Chu, Wengong Yu,