| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1390883 | Carbohydrate Research | 2007 | 7 Pages |
Abstract
Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl NI-acetyl-2II-azido-β-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a β-(1→4) linkage between the two disaccharides, while single mutant Cel7B E197A and triple mutant Cel7B E197A H209A A211T produce predominantly the β-(1→3)-linked tetrasaccharide. This result constitutes the first report of the modulation of the regioselectivity through site-directed mutagenesis for an endoglycosynthase.
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Authors
Sophie Blanchard, Sylvie Armand, Pedro Couthino, Shamkant Patkar, Jesper Vind, Eric Samain, Hugues Driguez, Sylvain Cottaz,