Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1391545 | Chemistry & Biology | 2014 | 10 Pages |
•IHR-1 is a Smo antagonist with limited cell permeability•Agonist-bound Smo or oncogenic forms of Smo signal from outside the cilium•A Smo-signaling complex in primary cilia is not required for oncogenic Smo signaling•The primary cilium may compartmentalize an endogenous Smo ligand
SummaryMisactivation of the seven-transmembrane protein Smoothened (Smo) is frequently associated with basal cell carcinoma and medulloblastoma. Cellular exposure to secreted Hedgehog (Hh) protein or oncogenic mutations in Hh pathway components induces Smo accumulation in the primary cilium, an antenna-like organelle with mostly unknown cellular functions. Despite the data supporting an indispensable role of the primary cilium in Smo activation, the mechanistic underpinnings of this dependency remain unclear. Using a cell-membrane-impermeable Smo antagonist (IHR-1), we demonstrate that Smo supplied with a synthetic agonist or activated with oncogenic mutations can signal without ciliary accumulation. Similarly, cells with compromised ciliary Smo trafficking due to loss of the phosphatidylinositol-4-phosphate 3-kinase (PI3K)-C2α retain transcriptional response to an exogenously supplied Smo agonist. These observations suggest that assembly of a Smo-signaling complex in the primary cilium is not a prerequisite for Hh pathway activation driven by Smo agonists or oncogenic Smo molecules.
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