Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1392534 | Chemistry & Biology | 2010 | 9 Pages |
SummaryWe determined the 2.2 Å crystal structures of the red fluorescent protein TagRFP and its derivative, the blue fluorescent protein mTagBFP. The crystallographic analysis is consistent with a model in which TagRFP has the trans coplanar anionic chromophore with the conjugated π-electron system, similar to that of DsRed-like chromophores. Refined conformation of mTagBFP suggests the presence of an N-acylimine functionality in its chromophore and single Cα-Cβ bond in the Tyr64 side chain. Mass spectrum of mTagBFP chromophore-bearing peptide indicates a loss of 20 Da upon maturation, whereas tandem mass spectrometry reveals that the Cα-N bond in Leu63 is oxidized. These data indicate that mTagBFP has a new type of the chromophore, N-[(5-hydroxy-1H-imidazole-2-yl)methylidene]acetamide. We propose a chemical mechanism in which the DsRed-like chromophore is formed via the mTagBFP-like blue intermediate.
Graphical AbstractFigure optionsDownload full-size imageDownload high-quality image (171 K)Download as PowerPoint slideHighlights► Crystal structure of the brightest monomeric red fluorescent protein TagRFP ► Crystal structure of the first blue fluorescent protein mTagBFP with tyrosine ► Chemical structure of a novel blue chromophore having the acylimine group ► Mechanism of formation of the DsRed-like red chromophore via a blue intermediate