| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1392841 | Chemistry & Biology | 2007 | 8 Pages |
Abstract
SummaryHere we report the ribosomal polymerization of α-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to reassign individual codons to seven types of α-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Atsushi Ohta, Hiroshi Murakami, Eri Higashimura, Hiroaki Suga,