DNA-binding study of anthraquinone derivatives using Chemometrics methods

This work presents the interaction of two anthraquinones including quinizarin (1, 4-dihydroxyanthraquinone) and danthron (1, 8-dihydroxyanthraquinone) with CT-DNA in a mixture of Brittone–Robinson buffer (pH = 7) with 50% of ethanol by UV–vis absorption, circular dichroism spectroscopic methods and viscosity measurements. The PCA and INDICES methods were used for predicting the number of light-absorbing components. Partially intercalative and hydrogen binding were found to be the dominant binding modes between anthraquinones and CT-DNA. The effect of ionic strength and pH on the behavior of the above system and also the interaction of anthraquinones with ds and ss-DNA were used to confirm the mentioned binding modes. The EQUISPEC software and nonlinear least-squares analysis were applied for binding constant determination.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Interaction of quinizarin and danthron with CT-DNA by chemometric methods was studied. ► The PCA and INDICES methods are used for predicting the number of components. ► Partially intercalative and H-binding are the dominant binding modes.