Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1393957 | Chemistry & Biology | 2009 | 5 Pages |
Abstract
SummaryPhotocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E. coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Francis B. Peters, Ansgar Brock, Jiangyun Wang, Peter G. Schultz,