| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1394026 | European Journal of Medicinal Chemistry | 2015 | 9 Pages |
•Interactions of OATP1B1 and OATP1B3 with antineoplastic compounds are studied in stably transfected HEK cells.•Vinblastine and paclitaxel strongly interact with OATP1B1.•Paclitaxel can contribute to OATP1B1 mediated drug–drug interactions in vivo.•Chlorambucil, mitoxantrone, vinblastine, vincristine, paclitaxel, and etoposide strongly interact with OATP1B3.•Mitoxantrone, paclitaxel and etoposide can contribute to OATP1B3 mediated drug–drug interactions in vivo.
Antineoplastic compounds are used in the treatment of a variety of cancers. The effectiveness of an antineoplastic compound to exert its activity is largely dependent on transport proteins involved in the entry of the compound into the cells, and those which drive it out of the cell. Organic anion transporting polypeptide 1B1 (OATP1B1) and organic anion transporting polypeptide 1B3 (OATP1B3), belonging to the SLCO family of proteins, are specifically expressed in the sinusoidal membranes of the liver, and are known to interact with a variety of drugs. The present study deals with the interaction of these proteins with antineoplastic compounds routinely used in cancer chemotherapy. The proteins OATP1B1 and OATP1B3 were functionally characterized in stably transfected human embryonic kidney cells using [3H] labeled estrone 3-sulfate and [3H] labeled cholecystokinin octapeptide (CCK-8) as substrates, respectively. Substrate uptake experiments performed in the presence of antineoplastic compounds showed that vinblastine and paclitaxel strongly interacted with the OATP1B1 with Ki values of 10.2 μM and 0.84 μM, respectively. OATP1B3 showed highly significant interactions with a variety of antineoplastic compounds including chlorambucil, mitoxantrone, vinblastine, vincristine, paclitaxel and etoposide, with Ki values of 40.6 μM, 3.2 μM, 15.9 μM, 30.6 μM, 1.8 μM and 13.5 μM, respectively. We report several novel interactions of the transporter proteins OATP1B1 and OATP1B3 highlighting the need to investigate their role in drug–drug interactions and cancer chemotherapy.
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