Amine–alkyl derivatives of hydantoin: New tool to combat resistant bacteria

A series of new 5,5-diphenylhydantoin derivatives with various amine–alkyl terminal fragments at N1-position were synthesized. Then a series of twenty-eight compounds with the same hydantoin scaffold were evaluated for their potency to combat bacterial MultiDrug Resistance (MDR). Intrinsic antibacterial activities were first evaluated. As these compounds showed no direct activity on bacteria, their influence on minimal inhibitory concentration (MIC) of nalidixic acid was tested in two strains of Enterobacter aerogenes: the reference-strain ATCC-13048 and the CM-64 strain which over-produces AcrAB-TolC efflux pump. The compounds showed moderate- or low- anti-MDR properties. According to SAR-studies, hit compounds containing 2-methoxyphenylpiperazine at N1-terminal fragment and methylcarboxyl acid one at N3-position of hydantoin have been identified for further microbiological studies and pharmacomodulations to develop efflux pump inhibitors.

Graphical abstract5,5-diphenylhydantoin derivatives with various amine–alkyl terminal fragments at N1-position were synthesized and evaluated for their potency to combat bacterial MultiDrug Resistance (MDR).Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Novel amine–alkyl derivatives of hydantoin 1–28 were synthesized. ► Antibacterial activity of hydantoin derivatives was tested in E. aerogenes. ► Potency of compounds to combat bacterial MultiDrug Resistance was investigated. ► Structure–activity relationship studies were performed.